Phospholamban overexpression in rabbit ventricular myocytes does not alter sarcoplasmic reticulum Ca transport

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Phospholamban overexpression in rabbit ventricular myocytes does not alter sarcoplasmic reticulum Ca transport.

Phospholamban has been suggested to be a key regulator of cardiac sarcoplasmic reticulum (SR) Ca cycling and contractility and a potential therapeutic target in restoring the depressed Ca cycling in failing hearts. Our understanding of the function of phospholamban stems primarily from studies in genetically altered mouse models. To evaluate the significance of this protein in larger mammalian ...

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Sarcoplasmic reticulum Ca release in intact ventricular myocytes.

Sarcoplasmic reticulum (SR) Ca release in intact ventricular myocytes is the major source of Ca which activates cardiac contraction (although Ca influx makes a non-negligible contribution in most species). The fundamental events of SR Ca release are known as Ca sparks. The twitch Ca transient is composed of approximately 10,000 Ca sparks occurring in a given cell, and they are synchronized by t...

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Effects of sarcoplasmic reticulum Ca -ATPase overexpression in postinfarction rat myocytes

Ahlers, Belinda A., Jianliang Song, JuFang Wang, Xue-Qian Zhang, Lois L. Carl, George M. Tadros, Lawrence I. Rothblum, and Joseph Y. Cheung. Effects of sarcoplasmic reticulum Ca -ATPase overexpression in postinfarction rat myocytes. J Appl Physiol 98: 2169–2176, 2005. First published January 27, 2005 ; doi:10.1152/japplphysiol.00013.2005.—Previous studies in adult myocytes isolated from rat hea...

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R-CEPIA1er as a new tool to directly measure sarcoplasmic reticulum [Ca] in ventricular myocytes.

In cardiomyocytes, [Ca] within the sarcoplasmic reticulum (SR; [Ca]SR) partially determines the amplitude of cytosolic Ca transient that, in turn, governs myocardial contraction. Therefore, it is critical to understand the molecular mechanisms that regulate [Ca]SR handling. Until recently, the best approach available to directly measure [Ca]SR was to use low-affinity Ca indicators (e.g., Fluo-5...

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Spatial and Temporal Inhomogeneities During Ca Release From the Sarcoplasmic Reticulum in Pig Ventricular Myocytes

The [Ca ]i transient of ventricular myocytes during normal excitation-contraction coupling is the summation of primary Ca release events, which originate at the junction of the sarcoplasmic reticulum (SR) and the T-tubular system. Studies in small mammals have shown a high density of release sites, but little is known of larger mammals. We have studied the spatial distribution of SR Ca release ...

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ژورنال

عنوان ژورنال: American Journal of Physiology-Heart and Circulatory Physiology

سال: 2009

ISSN: 0363-6135,1522-1539

DOI: 10.1152/ajpheart.00272.2008